SITEMAP

Contact STEMEXONE

ExoSort

Non-destructive High-purity Isolation Platform

Non-destructive ultra-high purity
exosome/cell isolation technology

The Challenge

Problems with existing methods

Forced dissociation

To separate the target from the magnetic beads, strong acidic solutions (pH 2-3) or strong physical rotational forces must be applied.

Critical drawbacks

Due to harsh conditions, the membranes of exosomes may be destroyed or cell viability may drastically decrease, leading to permanent damage to biological functions.

Capture
마그네틱 비드 + 엑소좀 강제 결합

Magnetic beads - forced binding of exosomes.

Forced separation
가혹한 조건

Harsh conditions

(Acidic solutions, physical force)

Damaged products
파괴된 막, 터진 모양

Damaged membranes, loss of function - permanent biological damage.

(Low recovery rate, low purity)

NeutraRelease principle

Gentle switch control

NeutraRelease is a 'reversible immunoseparation system' that controls the concentration of Ca2+ ions to autonomously release the binding loop. Since there is no chemical stimulation or physical shock, the original form of the target is preserved 100%.

1. Pre-activation

Attach anti-CBP antibodies to magnetic beads.

2. Immuno-bead

Calcium ion injection causes CBP and anti-CBP to bind.

3. Exosome capture

CBP-anti-CD63 binds to exosome CD63 for capture.

4. Recovery

Removal of calcium ions alters the shape of CBP, leading to exosome separation.

Cyclic measurement by repeating steps 1 to 4

Mechanism

01
Pre-activation

Activation and preparation of reversible linker (CBP)

02
Immuno-bead

Calcium switch ON: Target antibody binding

03
Capture

Capture target exosomes in liquid samples.

04
Recovery

Calcium switch OFF: Gentle dissociation and recovery

Comparison items Existing methods (such as MACS) NeutraRelease
Separation principle Strong acidic solution / physical force Calcium ion concentration control (neutral pH)
Condition of the product Risk of membrane destruction and loss of function Preservation of intact shape and function
Residual magnetic beads Possibility of bead residue on cell surfaces Bead-free (Clean Recovery)
Recovery rate and purity Loss due to harsh conditions Non-destructive high recovery rate and ultra-high purity
Core Competitive Advantages

100% maintenance of the shape and function of exosomes and cells.

The physical form, surface proteins, and internal core factors of exosomes are preserved intact without damage.

Non-destructive separation

Non-destructive separation

Maximizing analytical accuracy by perfectly preserving the physical properties of particles.

Ultra-high purity recovery

Ultra-high purity recovery

Selective and pure separation of cancer-specific exosome subtypes.

Bead-free state

Bead-free state

Perfect and clean results with no residual magnetic beads after recovery.

Revolutionary improvement in early diagnosis accuracy

Can accurately detect breast cancer, melanoma, prostate cancer, etc., at an early stage.

  • Detection of specific markers for breast cancer and melanoma
  • High-purity exosome separation for prostate cancer
  • Core technology of the liquid biopsy market

Promotion of cell therapy development

Non-destructive high-purity separation without reducing cell viability is an essential element of next-generation processes where living cells themselves become therapeutic agents. It facilitates the establishment of high-quality Master Cell Banks (MCB) and promotes the development of immune cell therapies through the selection of subtypes of NK cells and stem cells.

Innovation and value of technology

Based on differentiated technological capabilities, it was designated as an 'Innovative Product' by the Public Procurement Service in April 2024, securing numerous foundational patents both domestically and internationally, thereby establishing a unique technological barrier.